Journal: Journal of Medicinal Chemistry

Article Title: Structure-Based Design of Potent Bcl-2/Bcl-xL Inhibitors with Strong in Vivo Antitumor Activity

doi: 10.1021/jm300608w

Figure Lengend Snippet: Figure 7. (A) Representative cell growth inhibition of 12, 14, 15, and 1 in three small-cell lung cancer cell lines. (B) Cell death induction by 12, 14, 15, and 1 in H146 cell line. Cells were treated for 24 h, and cell death was analyzed by trypan blue assay. (C) Induction of cleavage of PARP and caspase-3 in H146 cell line by 12, 14, 15, and 1. Cells were treated for 24 h, and caspase-3 (Cas 3) and PARP were probed by Western blotting: Cl PARP, cleaved PARP; Cl Cas 3, cleaved caspase-3. GAPDH was used as the loading control.

Article Snippet: These assays were performed as previously described.17 Human smallcell lung cancer cell lines H146 and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in dx.doi.org/10.1021/jm300608w | J. Med.

Techniques: Inhibition, Western Blot, Control

Journal: Journal of Medicinal Chemistry

Article Title: Structure-Based Design of Potent Bcl-2/Bcl-xL Inhibitors with Strong in Vivo Antitumor Activity

doi: 10.1021/jm300608w

Figure Lengend Snippet: Figure 8. Analysis of cytochrome c (Cyto c) release from mitochondria into cytosol and cleavage of PARP (Cl PARP) induced by 14 and 15 in H146 cells in 2 h. GAPDH was used as the loading control.

Article Snippet: These assays were performed as previously described.17 Human smallcell lung cancer cell lines H146 and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in dx.doi.org/10.1021/jm300608w | J. Med.

Techniques: Control

Journal: Journal of Medicinal Chemistry

Article Title: Structure-Based Design of Potent Bcl-2/Bcl-xL Inhibitors with Strong in Vivo Antitumor Activity

doi: 10.1021/jm300608w

Figure Lengend Snippet: Figure 9. Analysis of induction of cleavage of PARP and caspase-3 in the H146 xenograft tumor tissues by 14 (25 mg/kg, iv). Mice bearing H146 xenograft tumors were dosed with either 14 or vehicle. Animals were sacrificed at 3, 6, and 24 h time points, and tumor tissues were analyzed by Western blot for cleavage of PARP (Cl PARP) and caspase-3 (Cl caspase-3). GAPDH was used as the loading control.

Article Snippet: These assays were performed as previously described.17 Human smallcell lung cancer cell lines H146 and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in dx.doi.org/10.1021/jm300608w | J. Med.

Techniques: Western Blot, Control

Journal: Journal of Medicinal Chemistry

Article Title: Structure-Based Design of Potent Bcl-2/Bcl-xL Inhibitors with Strong in Vivo Antitumor Activity

doi: 10.1021/jm300608w

Figure Lengend Snippet: Figure 10. Antitumor activity of compound 14 in H146 xenograft model. H146 tumor cells were injected subcutaneously into SCID mice, and treatments started when tumors reached a mean volume of 70 mm3. Each group consisted of eight mice/tumors.

Article Snippet: These assays were performed as previously described.17 Human smallcell lung cancer cell lines H146 and H1417 were purchased from American Type Culture Collection (ATCC) and were maintained in dx.doi.org/10.1021/jm300608w | J. Med.

Techniques: Activity Assay, Injection

Journal: Oncotarget

Article Title: Nicotinic acetylcholine receptors induce c-Kit ligand/Stem Cell Factor and promote stemness in an ARRB1/ β-arrestin-1 dependent manner in NSCLC

doi:

Figure Lengend Snippet: Microarray was performed in ARRB1 depleted and nicotine stimulated A549 cells Nicotine induced and ARRB1 dependent genes from the microarray data were analyzed. We identified differentially regulated genes that were regulated by nicotine in a β-arrestin-1 dependent fashion and top 10 up/down regulated genes from the list were used for prognosis prediction. Assessment of the expression of these genes for smoking revealed that SCF (highlighted in red) strongly differentiated smokers from non-smokers implying an important role of this gene in lung carcinogenesis induced by smoking

Article Snippet: A human lung cancer tissue microarray slide (Catalog number IMH-358; Imgenex, San Diego, CA) was immunostained for SCF.

Techniques: Microarray, Expressing, Control